ABSTRACT
ObjectiveTo prepare recombinant human basic fibroblast growth factor (rhbFGF) loaded magnesium-poly(D,L-lactide-co-glycolide) (Mg-PLGA) stent and to evaluate its angiogenesis effect in rat model of hindlimb ischemia.MethodsThe stent was prepared with spiral magnesium (Mg) and loaded with therapeutic agent rhbFGF and PLGA matrix.In vitro drug release study was carried out and the effect was evaluated using a standard animal model of rat hindlimb ischemia.A mechanical drill was conducted and the stent was implanted.The concentrations of Mg2+ in the muscle adjacent to the stent,rat plasma,urine and stools of the experimental animals were tested to analyze the degradation and metabolism of metal Mg.Immunohistochemical staining was performed to evaluate the angiogenesis effect of the stent.ResultsThe drug loaded in the stent could release continuously for about 4 weeks.The concentrations of Mg2+ in the rat plasma,urine and stools were within normal range.Immunohistochemical and quantitative analysis showed that the effect of Mg-PLGA-rhbFGF stent on angiogenesis of rat limb ischemia was better than that of the control group.ConclusionRhbFGF loaded MgPLGA stent could promote angiogenesis of rat limb ischemia,and it may provide theoretical basis for the critical patients suffered from lower limb ischemic disease.
ABSTRACT
[Objective] To evaluate the biocompatibility in vivo of Mg alloy AZ91D(AZ0),Mg-Mn-Ce(RE0)and their step anodizational materials(AZ1,AZ2,AZ3,RE1,RE2,RE3)by the experiments of intradermal stimulus and sensitivity response.[Methods]According toGB/T 16886.10-1997 Biological Evaluation of Medical Apparatus and Instrument,compared with normal sodium,pure magnesium and medical Ti-6Al-7Nb,the leaching liquor were injected into cutis to evaluate stimulus response,to evaluate sensitivity response by injection and illinition.[Results]RE2 could not prepare leaching liquor,it's kicked out.Intradermal stimulus response:normal sodium,Ti-6A1-7Nb,AZ1,AZ3 groups inflammation responded as degree I,scored 0.The others inflammation responded as degree Ⅱ,scored 1.Sensitivity response:formaldehyde group inflammation responded as degree IV,erythema and edema were degree IV.Normal sodium group inflammation responded as degree I,erythema and edema were degree 0.Ti-6Al-7Nb,AZ1,AZ3 groups inflammation responded as degree Ⅱ,erythema and edema were degree 1.The others inflammation responses were degree Ⅲ,erythema and edema were degree Ⅱ.[Conclusion]The new kinds of magnesium alloy AZ1,AZ3 possesses good preliminary biocompatibility in vivo,so it is very possible to be a new type of bone surgery implant material.